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Screening of biological activity of various species of the genus Narcissus sp. IV
Valešová, Zuzana ; Suchánková, Daniela (advisor) ; Vrabec, Rudolf (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Pharmacognosy and Pharmaceutical botany Author: Zuzana Valešová Supervisor: PharmDr. Daniela Suchánková, Ph.D. Title of thesis: Screening of biological activity of various species of the genus Narcissus sp. IV Key words: Narcisus sp., bulbs, alkaloidal extracts, GC/MS analysis, biological activity, acetylcholinesterase, butyrylcholinesterase Plants of the family Amaryllidaceae have a long history of using as traditional remedies. Plants of this family produce structurally unique isoquinoline compounds, which have been called "Amaryllidaceae alkaloids". Over 600 Amaryllidaceae alkaloids have been isolated, which are characterized by interesting biological activity. Cytotoxic, antimicrobial, antiparasitic, anti-inflammatory activity has been described, but the best known is the inhibitory activity against the enzymes acetylcholinesterase and butyrylcholinesterase, which is used in practice for therapy of Alzheimer's disease. [8, 9] The genus Narcissus is primarily known as decorative plants that blooms in early spring. However, daffodils are also used to isolate galanthamine or other alkaloids that are being studied for their effect. [13] The aim of this thesis was to screen the biological activity of extracts of the...
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Isolation of alkaloids of the species Geissospermum vellosii Allemão and study of their biological activity IV.
Emrichová, Eliška ; Šafratová, Marcela (advisor) ; Hradiská Breiterová, Kateřina (referee)
Eliška Emrichová: Isolation of alkaloids of the species Geissospermum vellosii Allemão and study of their biological activity IV. Diploma thesis 2020. Charles University, faculty of Pharmacy in Hradec Králové, Department of Pharmacognosy. Key words: Geissospermum vellosii, bark, alkaloidal extracts, isolation of alkaloids, GC/MS analysis, biological aktivity, acetylcholinesterase, butyrylcholinesterase. The aim of this study was to isolate at least one pure alkaloid from the extract of Geissospermum vellosii Alemão bark. The whole process involved bark processing, to obtain summary and alkaloid extract and subsequent column chromatography. GV-4, one of the 16 obtained fractions, was separated into 5 subfractions. The GV-4bsubfraction was used to isolate pure alkaloids, processed by preparative thin layer chromatography and crystallization of pure compound. The structure of pure compound was determined by using NMR, GC-MS analysis and optical rotation. This compound was identified as anhydropereirine and was tested its inhibitory activity against human cholinesterases, AChE and BuChE. The alkaloids GV-1-a, GV-8-3-B, GV-9-c were isolated in the course of further work on the extract. Their inhibitory activity against GSK-3β was tested as well as their possibility to cross the blood-brain-barieer with...
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Screening of biological activity of various species of the genus Narcissus sp. IV
Valešová, Zuzana ; Suchánková, Daniela (advisor) ; Vrabec, Rudolf (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Pharmacognosy and Pharmaceutical botany Author: Zuzana Valešová Supervisor: PharmDr. Daniela Suchánková, Ph.D. Title of thesis: Screening of biological activity of various species of the genus Narcissus sp. IV Key words: Narcisus sp., bulbs, alkaloidal extracts, GC/MS analysis, biological activity, acetylcholinesterase, butyrylcholinesterase Plants of the family Amaryllidaceae have a long history of using as traditional remedies. Plants of this family produce structurally unique isoquinoline compounds, which have been called "Amaryllidaceae alkaloids". Over 600 Amaryllidaceae alkaloids have been isolated, which are characterized by interesting biological activity. Cytotoxic, antimicrobial, antiparasitic, anti-inflammatory activity has been described, but the best known is the inhibitory activity against the enzymes acetylcholinesterase and butyrylcholinesterase, which is used in practice for therapy of Alzheimer's disease. [8, 9] The genus Narcissus is primarily known as decorative plants that blooms in early spring. However, daffodils are also used to isolate galanthamine or other alkaloids that are being studied for their effect. [13] The aim of this thesis was to screen the biological activity of extracts of the...
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Screening of biological activity of various species of the genus Kalanchoe
Mai, Thanh Tam ; Šafratová, Marcela (advisor) ; Hošťálková, Anna (referee)
Mai T. T.: Screening of biological activity of various species of the genus Kalanchoe, Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmacognosy and Pharmaceutical botany, Hradec Králové 2023. The content of this diploma thesis was the screening of the biological activity of dried flowers, stems and roots of four species from plants of the genus Kalanchoe: Kalanchoe delagoensis Eckl. & Zeyh., Kalanchoe fedschenkoi Raym.-Hamet & H.Perrier, Kalanchoe blossfeldiana Poelln. and Kalanchoe manginii 'Wendy' Raym.-Hamet & H.Perrier. By boiling the dried parts of the plants with ethanol, the summary ethanolic extracts were obtained, which were subjected to control TLC to detect the presence of alkaloids using Dragendorff's reagent. The presence of alkaloids was confirmed in the species Kalanchoe blossfeldiana Poelln, Kalanchoe manginii 'Wendy' Raym.-Hamet & H.Perrier and Kalanchoe delagoensis Eckl. & Zeyh. Furthermore, they were subjected to evidence reactions to identify the substances contained on TLC. In comparison with the literature, the presence of flavonoids, steroids substances, coumarins, triterpenes and alkaloids was confirmed in most plant extracts. Plants extracts of Kalanchoe blossfeldiana Poelln (AL-756 - extract from flowers and stems) and...
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Screening of biological activity of various species of the genus Narcissus sp. III
Růžičková, Zuzana ; Suchánková, Daniela (advisor) ; Chlebek, Jakub (referee)
CharlesUniversity,FacultyofPharmacyinHradecKrálové Department: DepartmentofPharmacognosyandPharmaceuticalbotany Author: Zuzana Růžičková Supervisor: PharmDr.DanielaSuchánková,Ph.D. Title of thesis: Screeningofbiologicalactivityofvariousspeciesofthe genusNarcissussp.III Key words: Narcissus sp., bulbs, alkaloidal extracts, GC/MS analysis, biological activity, acetylcholinesterase,butyrylcholinesterase Alzheimer'sdiseaseisaseverediseasethatrepresentsthemostcommoncauseofdementiaanddeath. Thedisease ischaracterized bya decrease off nerve cells and brain tissue.Forpatientswith Alzheimer's disease, memory lapses are typical, at the beginning of the disease states of anxiety and depression occur.The main cause isthe formation and deposition of β-amyloid and deficit of the neurotransmitter acetylcholine. Unfortunately, we do not know how to cure the Alzheimer's disease, but we can significantly slow it down by prolonging themild stages of the disease. Treatment is mainly directed to AChEinhibitorstoeliminateACHdeficiency. The main goal of this diploma thesis was to obtain summary alkaloid extracts from bulbs of seven cultivars of the genus Narcissus sp., which could have been a potential source of substances for the treatment of Alzheimer's disease. Cultivars were specifically Narcissus cv. Banana Splash,...
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Isolation of alkaloids of the species Geissospermum vellosii Allemão and study of their biological activity VII.
Strašková, Veronika ; Šafratová, Marcela (advisor) ; Hradiská Breiterová, Kateřina (referee)
Strašková, V.: Isolation of alkaloids of the species Geissospermum vellosii Allemão and study of their biological activity VII. Diploma thesis, Charles University, Pharmaceutical Faculty in Hradec Králové, Department of pharmacognosy and pharmaceutical botany, Hradec Králové 2023, 75 s. Key words: Geissospermum vellosii, bark, alkaloidal extracts, isolation of alkaloids, GC/MS analysis, biological activity, acetylcholinesterase, butyrylcholinesterase. The main goal of this diploma thesis was isolation of pure alkaloids from the stem bark of Geissospermum vellosii and test their biological activity. G. vellosii is a South American tree from the Apocynaceae family. It is used in traditional medicine for various pathological conditions (e.g., bacterial, inflammatory, and fungal diseases). Indole alkaloids isolated from the stem bark of this tree exhibit significant biological activities such as antineoplastic, antimalarial, and anticholinesterase activities. For processing, the GV-2, GV-7-D, and GV-4-F fractions were selected in this diploma thesis. These fractions were obtained in previous studies from the original alkaloid extract of the stem bark, which showed interesting inhibitory activity against AchE and BuChE (AChE: IC50 = 15,19±0,96 µg/ml, BuChE: IC50 = 0,37±0,049 µg/ml). Four alkaloids,...
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Isolation of alkaloids of the species Geissospermum vellosii Allemão and study of their biological activity IV.
Emrichová, Eliška ; Šafratová, Marcela (advisor) ; Hradiská Breiterová, Kateřina (referee)
Eliška Emrichová: Isolation of alkaloids of the species Geissospermum vellosii Allemão and study of their biological activity IV. Diploma thesis 2020. Charles University, faculty of Pharmacy in Hradec Králové, Department of Pharmacognosy. Key words: Geissospermum vellosii, bark, alkaloidal extracts, isolation of alkaloids, GC/MS analysis, biological aktivity, acetylcholinesterase, butyrylcholinesterase. The aim of this study was to isolate at least one pure alkaloid from the extract of Geissospermum vellosii Alemão bark. The whole process involved bark processing, to obtain summary and alkaloid extract and subsequent column chromatography. GV-4, one of the 16 obtained fractions, was separated into 5 subfractions. The GV-4bsubfraction was used to isolate pure alkaloids, processed by preparative thin layer chromatography and crystallization of pure compound. The structure of pure compound was determined by using NMR, GC-MS analysis and optical rotation. This compound was identified as anhydropereirine and was tested its inhibitory activity against human cholinesterases, AChE and BuChE. The alkaloids GV-1-a, GV-8-3-B, GV-9-c were isolated in the course of further work on the extract. Their inhibitory activity against GSK-3β was tested as well as their possibility to cross the blood-brain-barieer with...
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Isolation of alkaloids from Hippeastrum ferrari and their biological activity
Buchalová, Tereza ; Suchánková, Daniela (advisor) ; Hradiská Breiterová, Kateřina (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy and Pharmaceutical Botany Candidate: Tereza Buchalová Supervisor: PharmDr. Daniela Hulcová, Ph.D. Title of Thesis: Isolation of alkaloids from Hippeastrum cv. Ferrari and their biological activity Many different species, varieties, and cultivars of the genus Hippeastrum are popular indoor ornamental plants, but they also represent a rich source of important secondary metabolites called Amaryllidaceae alkaloids. These structurally unique compounds are known for their wide spectrum of biological activities such as anticancer, antiviral, and inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) which represent important part in therapy of Alzheimer's disease (AD). AD is a neurodegenerative disease that is identified as one of the most frequent causes of dementia in the world. Deficit of the neurotransmitter acetylcholine (ACh) in the cortex participates on the development of the AD, which results in the damage of cholinergic functions, and this is responsible for the memory loss and behavioural changes. AChE and BChE are enzymesinvolved in the termination of impulse transmission by rapid hydrolysis of ACh. Therefore, AChE and BChE inhibitors are nowadays the most important goal in...
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Isolation of alkaloids of the species Geissospermum vellosii Allemão and study of their biological activity VI.
Váňová, Lucie ; Šafratová, Marcela (advisor) ; Chlebek, Jakub (referee)
Lucie Váňová: Isolation of alkaloids of the species Geissospermum vellosii Allemão and study of their biological activity IV. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmacognosy and Pharmaceutical Botany, Hradec Králové 2022. Key words: bark, Geissospermum vellosii Allemão, alkaloids, preparative TLC, GC-MS, NMR, (+)-19,20-dehydrotubifolidine, aspidolimidine, geissoreticulatine, inhibition activity, AChE, BuChE, Alzheimer's disease The aim of this diploma thesis was the isolation of at least 3 alkaloids in pure form from the GV-5 fraction, which was extracted from the bark of the South American tree Geissospermum vellosii Allemão. By using preparative thin layer chromatography (TLC), 9 subfracions (GV-5-A až GV-5-I) were acquired from this fraction. Those that were of sufficient mass were subsequently subjected to further division. Three alkaloids in pure form were isolated through purification using preparative TLC: GV-5-B-3-A, GV-5-C-4-C, GV-5-C-2-B-1. Gained active substances from this plant were analyzed by gas chromatography- mass spektrometry (GC-MS) and nuclear magnetic resonance (NMR). By comparing the obtained alcaloid's spectra with available data and literature, those alkaloids were identified as: (+)-19,20-dehydrotubifolidine...
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Isolation of alkaloids of the species Geissospermum vellosii Allemão and study of their biological activity VIII.
Pelnář, Tomáš ; Šafratová, Marcela (advisor) ; Siatka, Tomáš (referee)
ISOLATION OF ALKALOIDS OF THE SPECIES GEISSOSPERMUM VELLOSII ALLEMÃO AND STUDY THEIR BIOLOGICAL ACTIVITY VIII. PELNÁŘ T. 1 , JANOUŠEK J. 2 , HRADISKÁ BREITEROVÁ K. 1 , ŠAFRATOVÁ M. 1 1 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy in Hradec Králové, Charles University in Prague, Czech Republic 2 Biomedical Research Center, University Hospital in Hradec Králové, Czech Republic e-mail: pelnart@faf.cuni.cz The aim of this work was to determine the activity of indole alkaloids, including their separation and purification from the South American tree Geissopsermum vellossi, which is used in traditional medicine to treat various diseases. It shows possible use in the treatment of benign prostatic hyperplasia, tumor treatment, plasmodial diseases, and especially cognitive disorders. The isolated structures were subsequently tested for inhibition of human cholinesterases.1 These substances could be used to treat Alzheimer's disease, which has been increasing in recent years. We are not yet able to stop disease, just slow it down. The starting product was a summary alkaloid extract from crushed tree bark, which showed very interesting activity against AChE and BChE (IC50 = 15,19±0,96 µg/ml for AChE and IC50 = 0,37±0,049 µg/ml for BChE). The GV-4 fraction has been processed using...
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